2GV8
Crystal structure of flavin-containing monooxygenase (FMO) from S.pombe and NADPH cofactor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.310, 72.530, 80.370 |
| Unit cell angles | 98.59, 107.40, 101.72 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vqw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.450 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.393 |
| Number of reflections | 68018 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 94.6 | 85.5 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG 3350, Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
