2GV5
crystal structure of Sfi1p/Cdc31p complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9797, 0.9799, 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.775, 104.735, 184.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.450 - 3.000 |
| R-factor | 0.25522 |
| Rwork | 0.253 |
| R-free | 0.29654 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.059 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.450 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.097 | 0.470 |
| Number of reflections | 34711 | |
| <I/σ(I)> | 5.4 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M sodium acetate, 18% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
