2GV0
The structure of the orthorhombic form of soft-shelled turtle lysozyme at 1.9 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Collection date | 2005-03-18 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.758, 55.561, 72.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.200 - 1.900 |
| Rwork | 0.207 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.327 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.069 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | |
| Number of reflections | 12433 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
