2GUZ

Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-30
Detector	MAR CCD 165 mm
Wavelength(s)	1.05
Spacegroup name	P 21 21 21
Unit cell lengths	111.591, 114.441, 162.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.20778
Rwork	0.205
R-free	0.25257
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.757
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.484
Number of reflections	137971
Completeness [%]	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.65M sodium citrate, protein concentration 400mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.65M sodium citrate, protein concentration 400mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K