2GUM
Crystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 |
Unit cell lengths | 84.959, 100.039, 100.133 |
Unit cell angles | 67.04, 77.99, 70.31 |
Refinement procedure
Resolution | 41.200 - 2.100 |
R-factor | 0.2479 |
Rwork | 0.248 |
R-free | 0.27470 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 | 0.331 |
Number of reflections | 145731 | |
<I/σ(I)> | 18.1 | 3.7 |
Completeness [%] | 0.9 | 0.588 |
Redundancy | 4.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% PEG 6000, 0.1 M Na Hepes, 15% 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |