2GUK
Crystal Structure of the Conserved Protein of Unknown Function from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.028, 50.028, 186.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.760 - 1.910 |
| R-factor | 0.23742 |
| Rwork | 0.233 |
| R-free | 0.28056 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.760 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.115 | 0.820 |
| Number of reflections | 19279 | |
| <I/σ(I)> | 10.5 | 2.2 |
| Completeness [%] | 98.6 | 96.7 |
| Redundancy | 20.7 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 275 | 5% iso-propanol, 0.1 M HEPES 7.5, 10 % PEG 4K, VAPOR DIFFUSION, SITTING DROP, temperature 275K |
