2GUJ
Three dimensional structure of the protein P54332 from Bacillus Subtilis. Northeast Structural Genomics Consortium target sr353.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97876, 0.97920, 0.96749 |
| Spacegroup name | P 32 |
| Unit cell lengths | 84.255, 84.255, 46.694 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.750 - 3.000 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 13213 | |
| Completeness [%] | 88.6 | 47.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 0.2M K, Na Tartrate, 2M Ammonium Sulphate, 0.1M Sodium Citrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP |
