2GUH
Crystal Structure of the Putative TetR-family Transcriptional Regulator from Rhodococcus sp. RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-20 |
Detector | SBC-3 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.259, 72.130, 133.875 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.370 - 1.520 |
R-factor | 0.17045 |
Rwork | 0.169 |
R-free | 0.18781 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.406 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.370 | 1.550 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.133 | 0.444 |
Number of reflections | 63688 | |
<I/σ(I)> | 9.6 | 3.9 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 9.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 25 % PEG3350, 0.2M MgCl2, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |