2GUG
NAD-dependent formate dehydrogenase from Pseudomonas sp.101 in complex with formate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8019 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.000, 54.980, 128.910 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.280 |
| R-factor | 0.1997 |
| Rwork | 0.196 |
| R-free | 0.26920 |
| Structure solution method | MOLREP |
| Starting model (for MR) | 2nac |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.862 |
| Data reduction software | MARHKL |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 16.100 |
| High resolution limit [Å] | 2.280 |
| Rmerge | 0.049 |
| Number of reflections | 73290 |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.8% PEG400, 2M Na formate, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
