2GUD
Crystal structure of a complex of griffithsin with mannose at 0.94 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.7 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.740, 39.440, 57.160 |
Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 0.940 |
R-factor | 0.1355 |
Rwork | 0.136 |
R-free | 0.15607 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gty |
RMSD bond length | 0.024 |
RMSD bond angle | 2.154 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.960 |
High resolution limit [Å] | 0.940 | 0.940 |
Number of reflections | 148669 | |
Completeness [%] | 95.5 | 75 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.8M magnesium sulfate,0.1M MES, 1:10 ratio of griffithsin monomers to mannose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |