2GU9
Crystal structure of XC5357 from Xanthomonas campestris: A putative tetracenomycin polyketide synthesis protein adopting a novel cupin subfamily structure
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97963, 0.96389 |
Spacegroup name | P 1 |
Unit cell lengths | 43.700, 43.680, 46.500 |
Unit cell angles | 65.00, 64.90, 73.40 |
Refinement procedure
Resolution | 30.000 - 1.400 |
R-factor | 0.231 |
Rwork | 0.220 |
R-free | 0.25300 |
Structure solution method | MAD |
RMSD bond angle | 0.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.460 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 32312 | |
Completeness [%] | 96.3 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M NaOAC, 1.6M LiSO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M NaOAC, 1.6M LiSO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |