2GU3
YpmB protein from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97872, 1.48520, 1.48590, 1.45180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.200, 52.415, 55.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.300 - 1.740 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21440 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.576 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 0.451 |
| Number of reflections | 12792 | |
| <I/σ(I)> | 25.5 | 2.44 |
| Completeness [%] | 98.4 | 88.6 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 10 mM nickel chloride, 25% PEG 2000 MME, 100 mM Tris buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
