2GTX

Structural Basis of Catalysis by Mononuclear Methionine Aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-09-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	49.809, 64.257, 76.172
Unit cell angles	90.00, 108.09, 90.00

Refinement procedure

Resolution	14.965 - 2.000
R-factor	0.204
Rwork	0.196
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xnz
RMSD bond length	0.005
RMSD bond angle	1.277
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.965	15.360	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.077	0.027	0.254
Total number of observations		3349	15854
Number of reflections	30975
<I/σ(I)>	9.5	20.9	3
Completeness [%]	99.8	92.3	100
Redundancy	3.6	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 20000, 0.1 M MES (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 291K