2GTL

Lumbricus Erythrocruorin at 3.5A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 1
Unit cell lengths	176.080, 257.960, 436.530
Unit cell angles	89.69, 97.15, 90.98

Refinement procedure

Resolution	100.000 - 3.500
R-factor	0.288
Rwork	0.288
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.078
Number of reflections	890862
Completeness [%]	89.3	74.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	6% (w/w) PEG 8000, 10mM CaCl2, 3% isopropanol, 60mM Hepes, pH 7.5, VAPOR DIFFUSION, temperature 298.0K