2GTE
Drosophila OBP LUSH bound to attractant pheromone 11-cis-vaccenyl acetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 95 |
Detector technology | CCD |
Wavelength(s) | 1.127 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.124, 65.270, 80.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.200 - 1.400 |
R-factor | 0.17768 |
Rwork | 0.175 |
R-free | 0.20283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Apo-LUSH |
RMSD bond length | 0.009 |
RMSD bond angle | 1.314 |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.078 | 0.311 |
Number of reflections | 55876 | |
<I/σ(I)> | 14 | 4.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.68 | 5.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1:1 protein/well sol. (4ul total) over 0.5 mL well, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |