Experimental procedure
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.250, 82.530, 54.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.110 |
R-factor | 0.165 |
Rwork | 0.165 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 22.920 * |
Data reduction software | MADNES |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | 2.210 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.084 | |
Total number of observations | 113811 * | |
Number of reflections | 21669 | |
Completeness [%] | 81.0 | 32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | glutathione sulphonate | 3 (mM) | |
3 | 1 | reservoir | PEG4000 | 16 (%(w/v)) |