2GSJ
cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-01 |
Detector | MAR scanner 180 mm plate |
Wavelength(s) | 1.43 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.190, 59.949, 76.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.310 - 1.730 |
R-factor | 0.1749 |
Rwork | 0.169 |
R-free | 0.19873 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.485 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.310 | 1.830 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.040 | 0.228 |
Number of reflections | 25805 | |
<I/σ(I)> | 20.8 | 8.1 |
Completeness [%] | 27.0 | |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M ammonium acetate, 0,1 M trisodium citrate dehydrate, pH 5.6 and 30% (w/v) PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |