2GSH
Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | P 2 3 |
Unit cell lengths | 135.145, 135.145, 135.145 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.393 |
R-factor | 0.19553 |
Rwork | 0.194 |
R-free | 0.24084 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gl5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.235 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.520 |
High resolution limit [Å] | 2.393 | 2.393 |
Rmerge | 0.085 | 0.562 |
Number of reflections | 32489 | |
<I/σ(I)> | 7.6 | 0.9 |
Completeness [%] | 99.6 | 97.2 |
Redundancy | 20.3 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 2.4M SODIUM MALONATE, 10% GLYCEROL, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 290K |