2GSC
Crystal Structure of the Conserved Hypothetical Cytosolic Protein Xcc0516 from Xanthomonas campestris
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9537, 0.97991, 0.97972 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.794, 104.257, 66.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.170 - 2.450 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.246 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.058 | 0.146 |
Number of reflections | 22715 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 98.5 | 98.9 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 200mM KCl, 5mM MgCl2, 50mM Na cacodylate pH 6.0, 16%(w/v) 1,6-hexanediol, 100mM CsCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |