2GS9

Crystal structure of TT1324 from Thermus thermophilis HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9, 0.97935, 0.97945
Spacegroup name	P 65 2 2
Unit cell lengths	161.662, 161.662, 139.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.490 - 2.600
R-factor	0.215
Rwork	0.215
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	JUPITER210 ((RIGAKU/MSC))
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	33701
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.4M Mg Formate, 0.1M Na Acetate (pH4.6), VAPOR DIFFUSION, HANGING DROP, temperature 293K