2GS8
Structure of mevalonate pyrophosphate decarboxylase from Streptococcus pyogenes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97908, 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.994, 72.065, 99.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 1.500 |
| R-factor | 0.16651 |
| Rwork | 0.165 |
| R-free | 0.19363 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.484 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 43511 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 0.1M sodium acetate trihydrate, 25% PEG 3350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
