2GS5
1.5 A Crystal Structure of a Conserved Protein of Unknown Function from Corvnebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.735, 74.914, 36.597 |
| Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
| Resolution | 37.450 - 1.500 |
| R-factor | 0.18493 |
| Rwork | 0.184 |
| R-free | 0.21024 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.083 | 0.236 |
| Number of reflections | 27257 | |
| <I/σ(I)> | 17.78 | 3 |
| Completeness [%] | 93.4 | 62.94 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.15M DL-malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
