2GS4
The crystal structure of the E.coli stress protein YciF.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-08-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794, 0.9797, 0.9252 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.870, 79.870, 131.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.490 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| Starting model (for MR) | RESOLVE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.199 |
| Number of reflections | 19196 | |
| <I/σ(I)> | 22.6 | 5.3 |
| Completeness [%] | 91.0 | 91.6 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1M citric acid pH 3.5-4.0, 1.9-2.1M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
