2GRZ
5ns Photoproduct of the M37V mutant of Scapharca HbI
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0-1.4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.040, 43.930, 83.440 |
Unit cell angles | 90.00, 121.95, 90.00 |
Refinement procedure
Resolution | 16.850 - 1.600 |
R-factor | 0.106 |
Rwork | 0.106 |
R-free | 0.10900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2grh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | Precognition |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.670 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 28081 | |
Completeness [%] | 73.8 | 33.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 7.5 | 298 | 57.5% Saturated Phosphate buffer, SMALL TUBES, temperature 298K |