2GRN
Crystal Structure of human RanGAP1-Ubc9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-31 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9790 |
Spacegroup name | P 1 |
Unit cell lengths | 39.326, 44.968, 61.260 |
Unit cell angles | 72.42, 72.09, 75.40 |
Refinement procedure
Resolution | 19.880 - 1.800 |
Rwork | 0.188 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.085 | 0.359 |
Number of reflections | 32808 | |
<I/σ(I)> | 9.3 | |
Completeness [%] | 93.5 | 86.5 |
Redundancy | 2.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |