2GRC

1.5 A structure of bromodomain from human BRG1 protein, a central ATPase of SWI/SNF remodeling complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	298
Detector technology	CCD
Collection date	2005-11-08
Detector	MARRESEARCH
Wavelength(s)	1.05, 0.9793, 0.9796, 0.9770
Spacegroup name	P 1 21 1
Unit cell lengths	29.860, 30.330, 66.820
Unit cell angles	90.00, 90.28, 90.00

Refinement procedure

Resolution	27.620 - 1.500
R-factor	0.24333
Rwork	0.242
R-free	0.26399
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.372
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.620	1.539
High resolution limit [Å]	1.500	1.500
Number of reflections	18402
Completeness [%]		99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	0.1 M Tris, 25% w/v PEG 3350, pH 8.5, VAPOR DIFFUSION, temperature 298.0K