2GRC
1.5 A structure of bromodomain from human BRG1 protein, a central ATPase of SWI/SNF remodeling complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-11-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05, 0.9793, 0.9796, 0.9770 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.860, 30.330, 66.820 |
Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
Resolution | 27.620 - 1.500 |
R-factor | 0.24333 |
Rwork | 0.242 |
R-free | 0.26399 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.372 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.620 | 1.539 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 18402 | |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 0.1 M Tris, 25% w/v PEG 3350, pH 8.5, VAPOR DIFFUSION, temperature 298.0K |