2GRB
Crystal Structure of an RNA Quadruplex Containing Inosine-tetrad
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Detector technology | CCD |
| Collection date | 2003-07-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9195,0.9200,0.8984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.060, 52.738, 37.344 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.19781 |
| Rwork | 0.195 |
| R-free | 0.25002 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.780 |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 23152 | |
| Completeness [%] | 95.3 | 71.5 |
| Redundancy | 6.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | KCl, CaCl2, MPD , pH 7.0, EVAPORATION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | CaCl2 | ||
| 7 | 1 | 2 | MPD |
