2GR3
Crystal structure of Ferredoxin reductase, BphA4 (oxidized form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.107, 98.107, 170.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.990 - 1.500 |
| R-factor | 0.19101 |
| Rwork | 0.190 |
| R-free | 0.20628 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.870 |
| Number of reflections | 75156 | |
| <I/σ(I)> | 23.49 | 2.7 |
| Completeness [%] | 96.3 | 89.3 |
| Redundancy | 18 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 2M Sodium formate, 0.1M Sodium acetate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
