2GQW
Crystal structure of Ferredoxin reductase, BphA4 (oxidized form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.409, 98.409, 170.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.17882 |
| Rwork | 0.178 |
| R-free | 0.19600 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.352 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 1342357 | |
| <I/σ(I)> | 22.8 | 5.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 2M Sodium formate, 0.1M Sodium acetate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
