2GPZ
Transthyretin-like protein from Salmonella dublin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-21 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 6 |
| Unit cell lengths | 94.705, 94.705, 57.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.920 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sn0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.193 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.017 | 2.630 |
| High resolution limit [Å] | 2.497 | 2.490 |
| Rmerge | 0.111 | 0.457 |
| Number of reflections | 10308 | |
| <I/σ(I)> | 6.5 | 1.6 |
| Completeness [%] | 99.5 | |
| Redundancy | 5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 295 | 20% PEG 3350, 0.2M magnesium chloride, 0.1M sodium cacodylate, pH 6.0, EVAPORATION, RECRYSTALLIZATION, temperature 295K, pH 6.00 |
