2GPR

GLUCOSE PERMEASE IIA FROM MYCOPLASMA CAPRICOLUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-11
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	40.330, 47.650, 71.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gpr
RMSD bond length	0.018
RMSD bond angle	20.200 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	TNT (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.063 *	0.162 *
Total number of observations	12034 *
Number of reflections	4952
<I/σ(I)>	28.3
Completeness [%]	97.0	85.3 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		PROTEIN WAS CRYSTALLIZED FROM 20-30% PEG 3000, 0.3MM ZNCL2, 100MM TRIS-HCL BUFFER, PH 7.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	20-26 (%)
2	1	reservoir		0.3 (mM)
3	1	reservoir	Tris-HCl	100 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	drop	Tris-HCl	10 (mM)