2GPE
Structure of the DNA-binding domain of E. Coli Proline Utilization A (PUTA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2006-03-09 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.2398 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.731, 55.731, 125.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.900 - 1.900 |
Rwork | 0.203 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ay0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.090 |
Data scaling software | d*TREK (9.4LDz) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.900 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.043 | 0.317 |
Total number of observations | 13007 | |
Number of reflections | 16253 | 1549 |
<I/σ(I)> | 25.5 | 5.7 |
Completeness [%] | 100.0 | |
Redundancy | 13.03 | 8.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.05M AMMONIUM SULFATE, 0.05M BIS-TRIS, 30% PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH). N-TERMINAL HIS TAG REMOVED BY TREATMENT WITH TEV PROTEASE , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |