2GPC
The crystal structure of the enzyme Fe-superoxide dismutase from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.626, 38.985, 91.073 |
| Unit cell angles | 90.00, 105.85, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.900 |
| R-factor | 0.15666 |
| Rwork | 0.154 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.118 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 27127 | |
| <I/σ(I)> | 10.6 | 2.7 |
| Completeness [%] | 94.6 | 69.9 |
| Redundancy | 4.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 24% PEG4000, 0.2 M MgCl2, 0.1 M TRIS-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
