2GPB

COMPARISON OF THE BINDING OF GLUCOSE AND GLUCOSE-1-PHOSPHATE DERIVATIVES TO T-STATE GLYCOGEN PHOSPHORYLASE B

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.181
RMSD bond length	0.016
RMSD bond angle	3.400
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.069 *
Total number of observations	85168 *
Number of reflections	34771 *
Completeness [%]	82.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7 *	unknown *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30 (mg/ml)
2	1	1	IMP	1 (mM)
3	1	1	spermine	1 (mM)
4	1	1	N,N-bis-[2-hydroxyethyl]-2-aminoethanesulfonic acid	10 (mM)
5	1	1	EDTA	0.1 (mM)
6	1	1	sodium azide	0.02 (%)