2GP5
Crystal structure of catalytic core domain of jmjd2A complexed with alpha-Ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Collection date | 2006-02-04 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.107, 149.524, 57.028 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.670 - 2.280 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.28500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.280 |
| Number of reflections | 32572 |
| Completeness [%] | 80.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% PEG 8000, 100 mM MgCl2, 100 Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
