2GP3

Crystal structure of the catalytic core domain of jmjd2a

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Collection date	2006-02-04
Wavelength(s)	0.9795 0.9793, 0.9600
Spacegroup name	P 21 21 2
Unit cell lengths	100.867, 149.540, 56.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.350
Rwork	0.247
R-free	0.28030
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.350	2.350
Number of reflections	33706
Completeness [%]	92.0	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	20% PEG8000, 200 mM MgCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K