2GOX
Crystal structure of Efb-C / C3d Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-11-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 41 |
| Unit cell lengths | 90.939, 90.939, 122.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.181 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3d |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.877 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.532 |
| Number of reflections | 49761 | 4664 |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 99.0 | 92.4 |
| Redundancy | 4.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 60% Tacsimate pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
