2GOT
Crystal structure of d(GCGAACGC): two types of bulge-containing duplexes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00, 0.91955, 0.92010 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 26.830, 26.830, 226.325 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.147 - 2.602 |
| R-factor | 0.254 |
| Rwork | 0.253 |
| R-free | 0.26900 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.654 |
| Data reduction software | DPS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.147 | 25.150 | 2.740 |
| High resolution limit [Å] | 2.602 | 8.220 | 2.600 |
| Rmerge | 0.054 | 0.041 | 0.266 |
| Total number of observations | 883 | 2074 | |
| Number of reflections | 1730 | ||
| <I/σ(I)> | 8.2 | 11.6 | 2.5 |
| Completeness [%] | 93.0 | 90.7 | 64.9 |
| Redundancy | 15.6 | 11.8 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 40mM sodium cacodylate, 12mM spermine tetrahydrochloride, 80mM sodium chloride, 10%(v/v) 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | spermine tetrahydrochloride | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | sodium chloride | ||
| 8 | 1 | 2 | HOH |
