2GOP

The beta-propeller domain of the Trilobed protease from Pyrococcus furiosus reveals an open velcro topology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.00, 1.10, 1.03
Spacegroup name	P 21 21 21
Unit cell lengths	54.747, 88.504, 153.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.720 - 2.000
R-factor	0.21014
Rwork	0.209
R-free	0.25824
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.134
Data reduction software	MOSFLM
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.720	2.107
High resolution limit [Å]	2.000	2.000
Number of reflections	39936
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	273	100 mM sodium acetate pH 4.4, 20 mM MgCl2, 300 mM 1,6-hexanediol, 10 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 273K
1	VAPOR DIFFUSION, HANGING DROP	4.4	273	100 mM sodium acetate pH 4.4, 20 mM MgCl2, 300 mM 1,6-hexanediol, 10 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 273K