2GON
Xray Structure of Gag133-278
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97895, 0.97858, 0.94002 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.260, 134.509, 42.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.900 |
| R-factor | 0.20493 |
| Rwork | 0.202 |
| R-free | 0.25713 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 48798 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 286 | 0.2 M BiAmmonium Citrate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
