2GOM
Crystal structure of Efb-C from Staphylococcus aureus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-08-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949, 0.97934, 0.97178 |
Spacegroup name | P 43 |
Unit cell lengths | 59.598, 59.598, 45.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.550 - 1.250 |
R-factor | 0.21095 |
Rwork | 0.211 |
R-free | 0.21804 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.928 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.063 | 0.544 |
Number of reflections | 44291 | |
<I/σ(I)> | 16.7 | 2.2 |
Completeness [%] | 86.9 | 99.2 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 3M Sodium Acetate pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |