2GO1
NAD-dependent formate dehydrogenase from Pseudomonas sp.101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.009 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 93.300, 93.300, 103.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.010 - 2.100 |
R-factor | 0.18923 |
Rwork | 0.186 |
R-free | 0.24224 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nac |
RMSD bond length | 0.020 |
RMSD bond angle | 1.737 |
Data reduction software | MARHKL |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.010 | 2.130 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.078 | 0.495 |
Number of reflections | 29590 | |
Completeness [%] | 94.7 | 91.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 290 | Ammonium Sulfate (48% saturated), 8% MPD, 0.1M HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |