2GNP
Structural Genomics, the crystal structure of a transcriptional regulator from Streptococcus pneumoniae TIGR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97907 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.044, 34.451, 71.627 |
Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
Resolution | 30.400 - 1.650 |
R-factor | 0.1956 |
Rwork | 0.193 |
R-free | 0.23677 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.782 |
Data reduction software | HKLSBC |
Data scaling software | HKL-2000 |
Phasing software | HKLSBC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.400 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.078 | 0.528 |
Number of reflections | 30578 | |
<I/σ(I)> | 30.39 | 2.57 |
Completeness [%] | 97.7 | 98.7 |
Redundancy | 4.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Sodium Acetate, 0.1M MES, 30% PEG 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |