2GNO
Crystal structure of a dna polymerase iii, gamma subunit-related protein (tm0771) from thermotoga maritima msb8 at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979224, 0.918370, 0.978940 |
Spacegroup name | I 4 |
Unit cell lengths | 135.560, 135.560, 35.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.940 - 2.000 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.684 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP (V. 1) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.900 | 2.070 | |
High resolution limit [Å] | 2.000 | 3.420 | 2.000 |
Rmerge | 0.041 | 0.025 | 0.415 |
Number of reflections | 22105 | 4199 | 3580 |
<I/σ(I)> | 11.88 | 25.1 | 2 |
Completeness [%] | 94.7 | 99.1 | 83.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6.2 | 277 | 35.0% 2-ethoxyethanol, 0.2M NaCl, 0.1M Na,K-Phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |