2GMY
Crystal Structure of a Protein of Unknown Function ATU0492 from Agrobacterium tumefaciens, Putative Antioxidant Defence Protein AhpD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.009, 75.009, 148.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.960 - 1.600 |
| R-factor | 0.16833 |
| Rwork | 0.167 |
| R-free | 0.19089 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.084 | 0.550 |
| Number of reflections | 116941 | |
| <I/σ(I)> | 15.74 | 1.44 |
| Completeness [%] | 99.7 | 98.88 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M NH4Sulphate, 2% PEG400, 0.1M Hepes Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
