2GMR
Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.850, 134.720, 141.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.430 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aig |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.433 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 50281 | |
| <I/σ(I)> | 24.49 | 6.35 |
| Completeness [%] | 97.4 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 301 | 20% PEG 4000, 100mM Tris-Cl, 750mM NaCl, 3% 1,2,3-heptanetriole, 0.12% LDAO, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 301.0K, pH 8.00 |
