2GM5
An activated, truncated gamma-delta resolvase tetramer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97180, 0.97930, 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.331, 98.476, 100.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.140 - 2.100 |
R-factor | 0.219 |
Rwork | 0.215 |
R-free | 0.25500 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.947 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.089 | 0.738 |
Number of reflections | 59517 | 5922 |
<I/σ(I)> | 7.7 | 1.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Protein in 200 mM NaCl, 3.75 mM DTT, 40 mM Tris-HCl, 0.05 M Li2SO4, 7.5% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |