2GM4
An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.138, 137.687, 83.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.28213 |
| Rwork | 0.280 |
| R-free | 0.32265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zr4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.091 | 0.759 |
| Number of reflections | 16189 | |
| <I/σ(I)> | 1.2 | |
| Completeness [%] | 96.5 | 84.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PROTEIN: DNA stock in 300 mM NaCl, 15 mM Tris-HCl, 0.05-0.15 M Mg Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
