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2GM4

An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X12B
Synchrotron siteNSLS
BeamlineX12B
Temperature [K]100
Detector technologyCCD
Collection date2003-07-16
DetectorADSC QUANTUM 315
Wavelength(s)1.05
Spacegroup nameP 21 21 2
Unit cell lengths110.138, 137.687, 83.473
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 3.500
R-factor0.28213
Rwork0.280
R-free0.32265
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1zr4
RMSD bond length0.008
RMSD bond angle1.157
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.630
High resolution limit [Å]3.5003.500
Rmerge0.0910.759
Number of reflections16189
<I/σ(I)>1.2
Completeness [%]96.584.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293PROTEIN: DNA stock in 300 mM NaCl, 15 mM Tris-HCl, 0.05-0.15 M Mg Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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