2GLX
Crystal Structure Analysis of bacterial 1,5-AF Reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9788 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.190, 84.900, 150.940 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 19.000 - 2.200 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.25100 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.615 |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.124 | 0.437 |
Number of reflections | 123108 | 14875 |
<I/σ(I)> | 8.56 | 3.6 |
Completeness [%] | 99.2 | 0.99 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 100mM NaCitrat, 200mM Ammoniumacetat, 30% MPEG 5000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 100mM NaCitrat, 200mM Ammoniumacetat, 30% MPEG 5000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |