2GLU
The crystal structure of YcgJ protein from Bacillus subitilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.899, 73.639, 245.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.910 |
| R-factor | 0.19686 |
| Rwork | 0.192 |
| R-free | 0.28257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxl |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.648 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13553 | |
| Completeness [%] | 94.6 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4M Am Sulfate, 0.1M Na Citrate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
